BHK cell variant with defective fibronectin receptor function |
| |
| Affiliation: | 1. Department of Pharmaceutical Technology and Chemistry, School of Pharmacy and Nutrition, University of Navarra, Irunlarrea 1, 31008 Pamplona, Spain;2. IDISNA-Instituto de Investigación Biosanitaria de Navarra, 31008 Pamplona, Spain;3. Department of Biochemistry & Genetics, School of Sciences, University of Navarra, Irunlarrea 1, 31008 Pamplona, Spain;4. Department of Environmental Biology, School of Sciences, University of Navarra, Irunlarrea 1, 31008 Pamplona, Spain;1. Ministry of Health, Manama, Bahrain;2. College of Medicine and Medical Sciences, Arabian Gulf University, Manama, Bahrain;3. Department of Clinical Nutrition and Dietetics, College of Health Sciences, Research Institute of Medical and Health Sciences (RIMHS), University of Sharjah, Sharjah, United Arab Emirates;1. School of Electrical Engineering, Yanshan University, Qinhuangdao, 066004, China;2. School of Electronic, Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China |
| |
| Abstract: | Baby hamster kidney cells were mutagenized with N-methyl-N′-nitro-N-nitrosoguanidine and selected to obtain a population of non-attaching cells. The cell variant FN-1 was cloned from the non-attaching cell population, recloned, and tested for cell adhesive interactions using four different assays of fibronectin (pFN) receptor function: cell attachment and spreading on culture dishes and cell binding and phagocytosis of latex beads. On pFN-coated culture dishes, FN-1 cells had decreased attachment compared to parental cells and were unable to spread. With pFN-coated beads, only one third as many pFN-bead binding sites could be detected on FN-1 cells as on the parental cells, and the FN-1 cells were unable to phagocytose the pFN-coated beads. In other studies, the variant cells were able to attach normally and spread partially on substrata coated with polycationic ferritin, concanavalin A, or anti-BHK cell surface antibody. The results suggest that the pFN-receptor function of FN-1 cells is defective. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
