Expression of cotransferred genes in mouse L cells |
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| Affiliation: | 1. Key Laboratory of Advanced Technologies of Materials, Ministry of Education, Chengdu 610031, China;2. School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, Sichuan 610031, China;3. Institut de Ciència de Materials de Barcelona (ICMAB-CSIC), Campus UAB, Bellaterra 08193, Barcelona, Spain;4. Key Laboratory of Infrared Imaging Materials and Detectors, Shanghai Institute of Technical Physics, Chinese Academy of Sciences, Shanghai 200083, China;5. Department of Chemistry and Physics, Fayetteville State University, Fayetteville, NC 28301, USA;1. School of Electrical and Electronic Engineering, 50 Nanyang Ave, Nanyang Technological University, 639798, Singapore;2. Energy Research Institute @ NTU (ERIAN), Interdisciplinary Graduate School, 50 Nanyang Ave, Nanyang Technological University, 639798, Singapore |
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| Abstract: | The Herpes simplex virus (HSV) thymidine kinase (TK) gene was introduced into mouse L cells by cotransformation with the G418 resistance gene on plasmid pSV2neo.d5 and selection for antibiotic resistance. Fifty nanograms each of the TK gene and pSV2neo.d5 were mixed with 10 μg of carrier DNA and precipitated onto cells. Of 18 antibiotic-resistant colonies analyzed, 15 contained 1–5 copies of the TK gene and 13 of the 15 were phenotypically TK positive. These data demonstrate that it is possible to introduce small numbers of copies of cotransferred genes into cultured cells and that in most cases the genes are integrated in a manner compatible with expression. |
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