Purine metabolism in cotyledons and embryonic axes of black gram (Phaseolus mungo L.) Seedlings |
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Affiliation: | Department of Food Science & Technology, University of Kashmir, Srinagar 190 006, India |
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Abstract: | - 1.1. The metabolism of purine bases and nucleosides in cotyledons and embryonic axes of black gram (Phaseolus mungo L.) was studied.
- 2.2. A large portion of absorbed [8-14C]adenine, [8-14C]guanine and [8-14C]adenosine was salvaged in nucleotide and nucleic acid synthesis.
- 3.3. Most of the radioactivity of [8-14C]hypoxanthine and [8-14C]inosine was incorporated into allantoin and allantoic acid.
- 4.4. Activity of adenine phosphoribosyltransferase in enzyme extracts was much higher than that of hypoxanthme and guanine phosphoribosyltransferase(s).
- 5.5. Apparent activity of adenosine kinase was higher than that of inosine kinase. 6. NAD+-dependent xan thine dehydrogenase was detected in both cotyledons and embryonic axes of the seedlings.
- 6.7. The capacity of purine salvage was higher m 24 hr old cotyledons than 24 and 48 hr old embryonic axes. The reverse was observed concerning that of purine degradation.
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