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Proteome analysis of a recombinant <Emphasis Type="Italic">Bacillus megaterium</Emphasis> strain during heterologous production of a glucosyltransferase |
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| Authors: | |
| Institution: | (1) TU-BCE, German Research Centre for Biotechnology, Mascheroder Weg 1, D-38124 Braunschweig, Germany;(2) Department of Structural Biology, German Research Centre for Biotechnology, Mascheroder Weg 1, D-38124 Braunschweig, Germany;(3) Institute of Microbiology, Technical University Braunschweig, Spielmannstrasse 7, D-38106 Braunschweig, Germany |
| Abstract: | A recombinant B. megaterium strain was used for the heterologous production of a glucosyltransferase (dextransucrase). To better understand the physiological and metabolic responses of the host cell to cultivation and induction conditions, proteomic analysis was carried out by combined use of two-dimensional gel electrophoresis and mass spectrometry (2-DE/MS) for protein separation and identification. |
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