Presence and distribution of histaminergic components in rat and bovine retina |
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| Institution: | 1. Instituto de Biología Fundamental “V. Villar Palasi” and Departamento de Bioquimica y Biologia Molecular, Universidad Autónoma de Barcelona, 08193 Bellaterra Barcelona, Spain;2. Departamento de Biologia Celular, Universidad Autónoma de Barcelona, 08193 Bellaterra Barcelona, Spain;1. Chemical Engineering Dep., College of Engineering, University of Tehran, 11155/4563, Tehran, Iran;2. Institute of Radiopharmaceutical Cancer Research, Helmholtz-Zentrum Dresden-Rossendorf (HZDR), Landstraße 400, 01328 Dresden, Germany;3. Institute of Process Engineering and Environmental Technology, TU Dresden, 01062 Dresden, Germany;4. Nanotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran 1417614411, Iran;1. Department of Neurology, F.M. Kirby Neurobiology Center, Boston Children’s Hospital, Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USA;2. Department of Neuroscience, Kavli Institute for Neuroscience, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA;1. Department of Animal Sciences and Marine Biology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran;2. Neuroscience Research Center and Department of Physiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran;3. Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran |
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| Abstract: | The presence of histamine and its related enzymes, histidine decarboxylase and histamine N-methyltransferase and the subcellular distribution of the amine and of H1-receptors were studied in the retina of two mammalian species. Histamine is present in rat and bovine retinas in concentrations (113 ± 10 and 72 ± 9 ng/g wet tissue, respectively) similar to those found in the brain. Histological examination and release experiments with Compound 48/80 performed in rat retina indicate a non mast cell location for the amine. Histidine decarboxylase and histamine N-methyltransferase activities in rat and bovine retinas were also comparable to those found in brain cortex suggesting that histamine can be synthesized and catabolyzed in situ. Subcellular fractionation of bovine retina showed that both the amine and H1-receptors are concentrated in particulate fractions where small sized synaptosomes sediment, presumably derived from horizontal and amacrine cells. These results are in agreement with a neurotransmitter or neuromodulator role for histamine in cells of the retinal inner nuclear layer. |
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