Effects of high KCI,isoproterenol, NaF and forskolin on noradrenaline release from cerebral cortical slices of adult and senescent rats |
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| Institution: | 1. Centro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR/CIMAR), Terminal de Cruzeiros do Porto de Leixões, Avenida General Norton de Matos S/N, 4450-208, Matosinhos, Portugal;2. Faculdade de Ciências da Saúde da Universidade Fernando Pessoa (FCS/UFP), Rua Calos Maia, 296, 4200-150, Porto, Portugal;3. Instituto Ciências Biomédicas Abel da Universidade do Porto (ICBAS/UP), Rua Jorge de Viterbo Ferreira 228, Porto, 4050-313, Portugal;4. Universidad de Las Palmas de Gran Canaria (ULPGC), Calle Juan de Quesada 30, 35001, Las Palmas de Gran Canaria, Las Palmas, Spain;5. Faculdade de Ciências da Universidade do Porto (FCUP), Rua Campo Alegre 1021/1055, 4169-007, Porto, Portugal;6. Faculdade de Farmácia da Universidade do Porto (FFUP), Laboratório Associado para a Química Verde (LAQV/REQUIMTE), Rua Jorge Viterbo Ferreira 228, 4050-313, Porto, Portugal;7. Universidade Federal de Pernambuco (UFPE), Departamento de Geologia, Núcleo de Estudos Geoquímicos e Laboratório de Isótopos Estáveis (NEG/LABISE), Recife, 7852, 50670-000, Brazil;1. Department of Biology, Temple University, Philadelphia, PA, United States;2. Department of Pediatrics, State University of New York, Downstate Medical Center, Brooklyn, NY, United States;3. Tisch Cancer Institute, Mount Sinai School of Medicine, New York, NY, United States;4. MIGAL-Galilee Technology Center, Cancer Drug Discovery Program, Kiryat Shmona, Israel;5. Department of Science, University Roma Tre, V.le G. Marconi, 446, 00146 Rome, Italy;6. Metabolomic Unit, CIC bioGUNE, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas, Technology Park of Bizkaia, Bizkaia, Spain;7. Department of Urology, Klinikum rechts der Isar der Technischen Universität München, Munich, Germany;8. Experimental Therapeutics and Translational Oncology Program, Instituto de Biología Molecular y Celular del Cáncer, CSIC/Universidad de Salamanca, Salamanca, Spain;9. Department of Medicine, University of Maryland School of Medicine, Baltimore, MD, United States;10. Department of Oncology, Johns Hopkins University School of Medicine and the Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD, United States;11. Tissue and Cell Culture Unit, CSIR-Central Drug Research Institute, Council of Scientific & Industrial Research, Lucknow, India;12. Department of Medicine, New York University Lutheran Medical Center, Brooklyn, NY, USA;13. Department of Microbiology, Biochemistry & Immunology, Morehouse School of Medicine, Atlanta, GA, United States;14. Department of Orthopedic Surgery, Nara Medical University, Kashihara 634-8521, Japan;15. Physics Department, School of Applied Mathematical and Physical Sciences, National Technical University of Athens, Zografou 15780, Athens, Greece;p. Institute of Cancer Sciences, University of Glasgow, UK;q. Department of Experimental and Clinical Medicine, University of Florence, 50134 Florence, Italy;r. Department of Biology, College of Science, UAE University, Al-Ain, United Arab Emirates;s. Department of Chemistry, College of Science, UAE University, Al-Ain, United Arab Emirates;t. Department of BioMedical Sciences, Creighton University, Omaha, NE, United States;u. School of Chemical and Bio Technology, SASTRA University, Thanjavur, India;v. Department of Biology, University of Rome “Tor Vergata”, 00133 Rome, Italy;w. Department of Research and Development, Ovarian and Prostate Cancer Research Trust Laboratory, Guildford, Surrey GU2 7YG, United Kingdom;x. Department of Comparative Pathobiology, Purdue University Center for Cancer Research, West Lafayette, IN, United States;y. Department of Pathology, Karmonas Cancer Institute, Wayne State University School of Medicine, Detroit, MI, United States;z. Brander Cancer Research Institute, Department of Pathology, New York Medical College, Valhalla, NY, United States;11. Mayo Graduate School, Mayo Medical School, Mayo Clinic Medical Scientist Training Program, Rochester, MN, United States;1. Department of Chemistry and Biochemistry, University of Maryland, 8051 Regents Drive, College Park, MD 20742, United States;2. Complex Carbohydrate Research Center, University of Georgia, Athens 30602, Georgia;1. Reckitt, Dansom Lane, Hull, HU8 7DS, United Kingdom;2. Department of Environmental Science, Radboud University Nijmegen, 6500GL, Nijmegen, the Netherlands;3. Ramboll Deutschland GmbH, Werinherstraße 79, 81541 München, Germany;1. Christian Doppler Laboratory for the Biotechnology of Skin Aging, Institute of Molecular Biotechnology, Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria;2. Christian Doppler Laboratory for Skin Multimodal Imaging of Aging and Senescence - SKINMAGINE, Vienna, Austria;3. Department of Dermatology, Medical University of Vienna, Vienna, Austria;4. Ludwig Boltzmann Research Group Senescence and Healing of Wounds, Vienna, Austria;5. Ludwig Boltzmann Institute for Experimental and Clinical Traumatology in AUVA Research Center, Vienna, Austria;6. Austrian Cluster for Tissue Regeneration, Vienna, Austria;7. Institute of Molecular Biotechnology, Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria |
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| Abstract: | Perfusion of isoproterenol (100 μM), 10mM NaF or 100 μM forskolin significantly enhanced release of l-3H]noradrenaline (NA) compared to the spontaneous release from cerebral cortical slices of adult rats. Isoproterenol-induced release was reduced by propranolol (100 μM). In the Ca2 + free medium and in the Ca2+ free medium containing 2.0 mM EGTA, the release enhanced by isoproterenol, NaF or forskolin was not different from that in the normal medium. In contrast, the marked reduction in 30 mM KCI-evoked release was observed in both Ca2+ -deficient media. It is suggested that β-adrenoceptor-stimulatory GTP-binding protein-adenylate cyclase system locating at presynaptic sites positively modulates NA release presumably by the mechanism other than increasing Ca2+ influxes. Furthermore, the cerebral cortical l-3H]NA uptake and the release induced by isoproterenol, NaF and forskolin as well as KCI and metamphetamine were significantly reduced at senescent stage compared to those at the adult stage, suggesting that presynaptic functions regarding the release as well as the uptake, could reduce with age. |
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