Acetoacetate,d-3-hydroxybutyrate and glucose utilization by capillaries isolated from developing rat brain |
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Affiliation: | INSERM U.26, Hôpital F. Widal, 200 rue du Fg. St-Denis, 75475 Paris, Cedex 10, France |
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Abstract: | Isolated cerebral capillaries from developing rats utilize glucose as well as ketone bodies essentially for oxidative metabolism. However, CO2 production from [U-14C]glucose was significantly greater than from ketone bodies (except at 5 mM). Ketone body utilization (in the presence of 5 mM glucose in the incubation medium) was concentration-dependent (up to 5 mM). Lipid synthesis from ketone bodies was comparable to that from glucose up to 1 mM. At concentrations ⩾ 1 mM, acetoacetate incorporation into total lipids and fatty acids was higher than other substrates, however, this difference was statistically significant only at 5 mM. Incorporation of substrates into sterols was very low (> 1 pmol/h/mg protein). |
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