Marker enzymes,phospholipids and acyl group composition of a somal plasma membrane fraction isolated from rat cerebral cortex: a comparison with microsomes and synaptic plasma membranes |
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| Institution: | 1. Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot 7610001, Israel;2. Department of Veterinary Resources, Weizmann Institute of Science, Rehovot 7610001, Israel;1. Department of Pharmacy, Xiangya Hospital, Central South University, Changsha 410008, China;2. Institute of Hospital Pharmacy, Xiangya Hospital, Central South University, Changsha, 410008, China;3. Key Laboratory of Hunan Province for Traditional Chinese Medicine in Obstetrics and Gynecology Research, Hunan Provincial Maternal and Child Health Care Hospital, No. 53 XiangChun Road, Changsha 410008, China;4. Department of Neurology, Xiangya Hospital, Central South University, Changsha 410008, China;5. Institute of Clinical Pharmacy, Jining First People’s Hospital, Jining Medical University, Jining 272000, China;1. Department of Medical Neurobiology, Institute for Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School, Jerusalem, Israel;2. Department of Psychiatry, Hadassah-Hebrew University Medical Center, Jerusalem, Israel;3. Department of Microbiology and Molecular Genetics, Institute for Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School, Jerusalem, Israel;1. Institute for Health and Biomedical Innovation, Queensland University of Technology, Brisbane 46000, Australia;2. Institute for Macromolecular Chemistry, Albert-Ludwigs-Universität, Freiburg 79114, Germany;1. Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China;2. College of Agronomy, Anhui Agricultural University, Hefei 230036, China;3. Agricultural Bureau of Deqing, Huzhou 313000, China;1. Department of Psychiatry and Psychotherapy, Jena University Hospital, Jena, Germany;2. Department of Neurology, Jena University Hospital, Jena, Germany |
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| Abstract: | Subjecting brain homogenates to differential speed and sucrose density gradient centrifugation resulted in the isolation of a membrane fraction from the post-mitochondrial supernatant with properties and marker enzyme profiles typical of plasma membranes. This membrane fraction is compared with the microsomes and the synaptic plasma membranes isolated from synaptosomes. Like the synaptic plasma membranes, membranes obtained from the post-mitochondrial supernatant were enriched five-fold in 5′-nucleotidase activity. However, the latter membranes were lower in (Na+, K+)-ATPase activity and higher in NADPH-cytochrome C reductase activity as compared to the synaptic plasma membranes. The post-mitochondrial plasma membranes were also different from the microsomes in their respective marker enzyme activities. Electron microscopic examination indicated largely membranous vesicles for both plasma membrane fractions with little contamination by myelin, mitochondra and intact synaptosomes. The phospholipid and acyl group profiles of the two plasma membrane fractions were surprisingly similar, but they were different from the characteristic profiles of myelin and mitochondria. It is concluded that plasma membranes isolated from the post-mitochondrial supernatant fraction are derived largely from neuronal and glial soma and are thus designated the somal plasma membrane fraction. |
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