Na+ K+ ATPase activity in synaptosomes and myelin of developing control and intra-uterine growth retarded rats: effects of lead and serotonin |
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| Institution: | 1. INSERM Unité 29, Hôpital Port-Royal, 123 Boulevard de Port-Royal, 75014 Paris, France;2. INSERM Unité 26, Hôpital Fernand Widal, 200 rue du Faubourg Saint-Denis, 75475 Paris, France;1. Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China;2. University of Chinese Academy of Sciences, Beijing 100049, China;1. Department of Pharmacology, Basic Medical School of Wuhan University, Wuhan, 430071, China;2. Department of Orthopaedic Surgery, Zhongnan Hospital of Wuhan University, Wuhan, 430071, China;3. Hubei Provincial Key Laboratory of Developmentally Originated Disease, Wuhan, 430071, China;1. Programa de Pós Graduação em Neurociências, Instituto de Ciências Básicas, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil;2. Programa de Pós Graduação em Bioquímica, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil;3. Programa de Pós Graduação em Saúde da Criança e do Adolescente, Faculdade de Medicina, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil;4. Programa de Pós Graduação em Psiquiatria, Faculdade de Medicina, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil;5. Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil;6. Ludmer Centre for Neuroinformatics and Mental Health, Douglas Mental Health University Institute, McGill University, Montreal, QC, Canada;7. Dept of Ob/Gyn, Harbor-UCLA Med Ctr, Torrance, CA, United States;8. Department of Psychiatry, Faculty of Medicine, McGill University, Sackler Program for Epigenetics & Psychobiology at McGill University, Montreal, QC, Canada;1. Programa de Pós-Graduação em Saúde da Criança e do Adolescente. Faculdade de Medicina, Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2350, Largo Eduardo Zaccaro Faraco, CEP 90035-903 Porto Alegre, RS, Brazil;2. Programa de Pós-Graduação em Neurociências. Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite, 500, Sala 107 (Campus Centro), CEP 90046-900 Porto Alegre, RS, Brazil;3. Department of Obstetrics and Gynecology, Harbor-UCLA Medical Center, 1124 West Carson Street, Box 446, RB-1 Bldg., Torrance, CA 90502, USA |
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| Abstract: | An intrauterine growth retarded (IUGR) model based on restriction of blood supply to fetuses at 17 days of pregnancy in rats was studied. We investigated in vitro the effects of lead on Na+K+ ATPase activity in synaptosomes and myelin of IUGR and control rats from 6 to 60 days after birth. In both groups an age-dependent effects existed in synaptosomes for the lowest doses of lead. The experimental group tended to be more sensitive to the metal than the control group and the Na+K+ATPase activity was less inhibited in the younger rats as compared to mature rats. Serotonin (5-HT) added to the subcellular preparations produced different changes in Na+K+ATPase activity. In synaptosomes, 5-HT stimulated the enzyme activity in a dose-related manner and apparently reversed the inhibiton induced by lead up to 22 days after birth in the control group. This action was less marked in the IUGR group. In myelin fractions, the Na+K+ATPase activity was inhibited by lead in both groups but the “protective effect of monoamines” was never observed. The Na+K+ ATPase activity was modulated by monoamines in synaptosomes and not in myelin, perhaps through a mechanism involving soluble factor(s). |
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