Lamprey brain contains globular and asymmetric forms of acetylcholinesterase |
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| Institution: | 1. Center for Mathematical Science and Advanced Technology, Research Institute for Value-Added-Information Generation, Japan Agency for Marine-Earth Science and Technology, 3173-25 Showa-machi, Kanazawa-ku Yokohama, Japan;2. Subduction Dynamics Research Center, Research Institute for Marine Geodynamics, Japan Agency for Marine-Earth Science and Technology, 3173-25 Showa-machi, Kanazawa-ku Yokohama, Japan;3. Kobe University, Department of Planetology, Graduate School of Science, 1-1 Rokkodai, Nada-ku, Kobe, Japan;1. Institute of Health and Wellbeing, Mt Helen Campus, Federation University Australia, Ballarat, VIC 3350, Australia;2. Successful Health for At-Risk Populations (SHARP) Research Team, Mt Helen Campus, Federation University Australia, Australia;3. Institute of Health and Wellbeing, Churchill Campus, Federation University Australia, Churchill, VIC 3842, Australia;4. Health Innovation and Transformation Centre, Mt Helen Campus, Federation University Australia, Ballarat, VIC 3350, Australia;5. Institute for Health and Sport, Victoria University, Footscray, VIC 3011, Australia |
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| Abstract: | To obtain more information about the evolution of acetylcholinesterase in the vertebrates, we studied the cholinesterase activity from the brain of the lamprey Petromyzon marinus. We found that the enzyme is true acetylcholinesterase and that 98% of it is present in the G4 globular form. Only 1% of the enzyme was found distributed among the asymmetric forms A4, A8 and A12; an additional 1% of the activity could not be extracted from the brain. The identity of the asymmetric forms was confirmed by collagenase digestion. These data demonstrate that asymmetric acetylcholinesterase is present in the CNS of organisms representing all classes of vertebrates. However, our results are inconsistent with an evolutionary trend that has been observed for vertebrate brain acetylcholinesterase. |
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