基于遗传算法的光合细菌过氧化物酶电泳分析方法的建立

建立光合细菌中过氧化物酶的酸性聚丙烯酰胺凝胶电泳(A-PAGE)分析方法。以电泳图谱的谱带数和灰度为指标, 运用正交试验和遗传算法对该方法的电泳条件进行优化。实验结果表明, 当凝胶中Triton X-100含量为0.31%、菌体破碎时间为19 min、样品中Triton X-100含量为0.79%时, 可获得清晰的沼泽红假单胞菌过氧化物酶图谱; 当凝胶中Triton X-100含量为0.27%、菌体破碎时间为16 min、样品中Triton X-100含量为0.76%时, 可获得清晰的球形红细菌过氧化物酶图谱。本方法为光合细菌中过氧化物酶的分析提供了一种新的、简单、快速的手段。

Establishment of Gel Electrophoresis for the Analysis of Peroxidases in Photosynthetic Bacteria Based on Genetic Algorithm

To establish the acid polyacrylamide gel electrophoresis (A-PAGE) analyzing method of peroxidase isoenzymes in photosynthetic bacteria. According to the number and the grey scale of bands, the conditions of the A-PAGE were optimized by the orthogonal design and the genetic algorithm. The results indicated that when the Triton X-100 concentration in the gels was 0.31%, the ultrasonic time was 19 min, the Triton X-100 concentration in the samples was 0.79%, the clear peroxidase isoenzyme zymograms of Rhodopseudomonas palustris were obtained, when the Triton X-100 concentration in the gels was 0.27%, the ultrasonic time was 16 min, the Triton X-100 concentration in the samples was 0.76%, the clear peroxidase isoenzyme zymograms of Rhodobacter sphaeroides were obtained. The paper set up a simple and rapid method for the analysis of peroxidase isoenzymes in photosynthetic bacteria.