Plant regeneration from leaf discs of peanut and pigeonpea: Influence of benzyladenine,indoleacetic acid and indoleacetic acid-amino acid conjugates

The influence of indoleacetic acid (IAA) and some IAA-amino acid conjugates in combination with benzyladenine (BA) on in vitro shoot regeneration from leaf discs of peanut and pigeonpea was investigated. The frequency of shoot regeneration and average number of shoot buds produced was dependent on the type of auxin present in the medium. Highest frequency of plant regeneration in peanut was induced by BA in combination with IAA or IAA-l-alanine, while in pigeonpea BA in combination with IAA or IAA-l-aspartic acid produced best results. Four hundred plants of peanut and 25 plants of pigeonpea were transplanted to soil.Abbreviations BA benzyladenine - IAA indoleacetic acid - IAA l-alanine-indole acetyl-l-alanine - IAA aspartic acid-indole acetyl-l-aspartic acid - IAA glycine-indole acetyl-l-glycine - IAA phenylalanine-indole-acetyl-l-phenyl-alanine