|
|||||
|
|
A semi-integrated method for the determination of enzyme kinetic parameters and graphical representation of the Michaelis-Menten equation |
|
| Authors: | A Naqui B Chance |
| Affiliation: | 1. Department of Biochemistry and Biophysics, Richards Bldg D-501, University of Pennsylvania, Philadelphia, Pennsylvania 19104 USA;2. Institute for Structural and Functional Studies, University City Science Center, 3401 Market Street, Philadelphia, Pennsylvania 19104 USA |
| Abstract: | A semi-integrated method for the determination of the enzyme kinetics parameters (Km and V) and graphical representation of the Michaelis-Menten equation is proposed as a variation of determination of initial reaction rate (v) as a function of initial substrate concentration ([S]0). The method is based on the determination of the time required to exhaust half of the initial substrate concentration as a function of the initial substrate concentration. The advantages and limitations of this method are discussed. |
| Keywords: | Michaelis-Menten equation enzyme kinetics half-time |
| 本文献已被 ScienceDirect 等数据库收录！ | |