双色荧光杂交芯片在检测 P 1A 1M P I 基C 因多态性中的应用

目的：应用一种新的高通量SNP检测方法-双色荧光杂交芯片技术检测CYPIA1 MspI基因多态性。方法：收集江苏汉族人群原发性肺癌患者75例和相应对照77例，应用双色荧光杂交芯片技术检测了152例样本的CYPIAI基因MspI基因多态性，并应用PCR-RFLP技术验证双色荧光杂交芯片的特异性。结果：152例样本的CYPIAI基因双色荧光杂交芯片技术分型结果与PCR-RFLP结果完全相符，两种方法的基因型分型结果具有很好的一致性。结论：双色荧光杂交芯片技术是一个高通量SNP检测的良好工具，特异性高，在大规模人群SNP筛检中具有良好的发展前案。

Application of Dual-color Fluorescence Hybridization chip Technique in the detection of CYP1A1 MspI genetic polymorphism

Objective: To detect CYP1A1 MspI genetic polymorphism by a new high-throughput single nucleotide polymorphism (SNP) typing approach of dual-color fluorescence hybridization chip technique. Methods: 75 patients with lung cancer and 77 cancer-free controls were collected to detect CYP1A1 MspI polymorphism. Genotypes were analyzed by dual-color fluorescence hybridization chip technique and PCR-RFLP, respectively. The results of dual-color fluorescence hybridization chip were compared with PCR-RFLP to evaluate the genotyping validity. Results: The genotying results of dual-color fluorescence hybridization chip on 152 samples matched the PCR-RFLP results. Conclusion: Dual-color fluorescence hybridization chip is a specific and high-throughput tool in SNP genotyping,which has a favorable applied foreground for SNP genotyping in a large population.