鲤胚胎孵化腺细胞

鲤胚胎孵化腺为单细胞腺体,发生于外胚层,可特异地被PAS染色。最早可在眼色素期检验出孵化腺细胞(Hatching gland cell,HGC)它们主要分布在头部腹面及头部与卵黄囊连接处。开始,HGC位于表皮细胞下面,随发育迁移到胚胎表面。根据扫描和透射电镜观察,在分泌孵化酶的前后,HGC区表面细胞呈鸡冠花状和疣状两种突起。前者系HGC处于分泌孵化酶期间;后者系HGC业已完成分泌作用,由于相邻的表皮细胞活动而形成的。HGC内富有粗面内质网、线粒体、核糖体和高尔基体,并由后者合成酶原颗粒。HGC在完成分泌作用后,仍留在表皮中,以后逐渐退化,但在孵化后30h仍可见残留的HGC。

THE HATCHING GLAND CELL OF CARP (CYPRJNUS CARPIO) EMBRYO

The hatching gland of the carp embryo was an unicellular gland. It originated from the ectoderm. The earlist stage at which the hathing gland cell (HGC) can be detected with PAS specific staining was the eye-pigmented stage. The HGCs were majorly located in the ventral part of the head and in the jointing region between head and yolk sac. Oringinally, HGCs were beneath the epidermal cells. During development, they migrated to the surface of the embryo. Based on observations with SEM and TEM, the epidermal cells in HGC region had two kinds of processes, one cockscomb-like and the other wartlike before and after the enzyme secretion. The former was present before and during the period of secretion; the latter appeared after the secretion period, and accounted for the movement of the epidermal cells in this region. HGCs were rich in RER, mitochondria, ribosomes and Golgi complexes in which the proenzyme granules were formed. After the period of enzyme secretion, HGCs still remained in the epithelium and gradully degenerated with the development of the embryo. However, they could still be seen 30 hours after hatching.