Identification and immunohistochemical localization of various bovine pancreatic trypsin inhibitor-isoforms in bovine pituitary gland |
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Authors: | Laura Fiorucci Gabriella De Renzis Rita Businaro Lorenzo Fumagalli Evandro Fioretti Bruno Giardina and Franca Ascoli |
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Institution: | (1) Department of Experimental Medicine and Biochemical Sciences, University Tor Vergata, via O. Raimondo, 00173 Rome, Italy;(2) Department of Anatomy, Catholic University, via della Pineta Sacchetti 644, 00168 Rome, Italy;(3) Department of Neurological Sciences, University La Sapienza, P. Aldo Moro, 00185 Rome, Italy;(4) Department of Cell Biology, University of Camerino, Via Scalzino, 62032 Camerino, Italy |
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Abstract: | Summary Three isoinhibitors of bovine pancreatic trypsin inhibitor (BPTI) have been identified and isolated from bovine pituitary gland. The results of the purification process by affinity chromatography on immobilized trypsin, the electrophoretic mobility in non-denaturing conditions, the antiproteolytic activity and the immunochemical reactions indicate that these inhibitors correspond to those previously isolated from bovine spleen and lung. In addition, immunohistochemical experiments show that the isoinhibitors and BPTI are exclusively localized in the mast cells, and not in the endocrine cells, of the pars intermedia and posterior lobe (neurohypophysis) of the pituitary gland. The physiological implications of these findings are discussed. |
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