Effect of annexin I on insulin secretion through surface binding sites in rat pancreatic islets |
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Authors: | Hong Shin-Hee Won Jong Hak Yoo Seung-Ah Auh Chung-Kyoon Park Young Min |
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Affiliation: | Department of Biological Sciences, SungKyunKwan University, Suwon 440-746, South Korea. |
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Abstract: | This study investigates the effect of extracellular annexin I (Anx I) on regulating insulin secretion in isolated rat pancreatic islets. Results show that Anx I stimulates insulin release in pancreatic islets regardless of the presence or absence of extracellular Ca2+. In particular, confocal microscopy shows that Anx I binds to the surface of islet cells in the absence of extracellular Ca2+. However, insulin secretion through Anx I significantly decreases in trypsin-treated islets. Likewise, there is minimal binding of Anx I to the surface of trypsin-treated islets. Anti-Anx I polyclonal antibody also inhibits the stimulating effect of Anx I on insulin secretion. These results indicate that Anx I is capable of binding to the cell surface receptor, in order to regulate the stimulation of insulin release in rat pancreatic islets. |
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