Isolation and Identification of Natural Endophytic Rhizobia from Rice (Oryza sativa L.) Through rDNA PCR-RFLP and Sequence Analysis |
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| Authors: | Ramesh K. Singh Ravi P. N. Mishra Hemant K. Jaiswal Vinod Kumar Shree P. Pandey Sasi B. Rao Kannepalli Annapurna |
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| Affiliation: | (1) Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, 221005, India;(2) Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, 221005, India;(3) Microbiology Division, Indian Agricultural Research Institute, New Delhi, India;(4) Present address: Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Hans-Knoll-Stresse 8, Jena, Germany |
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| Abstract: | Three novel endophytic rhizobial strains (RRE3, RRE5, and RRE6) were isolated from naturally growing surface-sterilized rice roots. These isolates had the ability to nodulate common bean (Phaseolus vulgaris). Polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) and sequencing of 16S rDNA of these isolates revealed that RRE3 and RRE5 are phylogenetically very close to Burkholderia cepacia complex, whereas RRE6 has affinity with Rhizobium leguminosarum bv. phaseoli. Plant infection test using gusA reporter gene–tagged construct of these isolates indicated that bacterial cells can go inside and colonize the rice root interiors. A significant increase in biomass and grain yield was also recorded in greenhouse-grown rice plants inoculated with these isolates. |
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