Isolation and characterization of a mutant of Saccharomyces cerevisiae defective in phosphoenolpyruvate carboxykinase |
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Authors: | Perea Javier Gancedo Carlos |
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Affiliation: | (1) Instituto de Enzimologia del C.S.I.C., Facultad de Medicina de la Universidad Autónoma, Madrid 34, Spain |
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Abstract: | A mutant of Saccharomyces cerevisiae lacking phosphoenolpyruvate carboxykinase (E.C. 4.1.1.32) was isolated. The mutant did not grow on gluconeogenic sources except glycerol. The mutation was recessive and apparently affected the structural gene of the enzyme. Intracellular levels of metabolites related to the metabolic situation of the enzyme were not significantly affected after transfer of the mutant from a medium with glycerol to a medium with ethanol as carbon source. In these conditions only AMP decreased 3 to 5 times. A search for mutants affected in the other gluconeogenic enzyme, fructose 1,6 bisphosphatase, remained unsuccessful.Abbreviation PEPCK phosphoenolpyruvate carboxykinase (E.C. 4.1.1.32) |
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Keywords: | Phosphoenolpyruvate carboxykinase Gluconeogenesis Saccharomyces cerevisiae Mutant |
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