Genetic and enzymatic characterization of a conditional lethal mutant of Escherichia coli K12 with a temperature-sensitive DNA ligase |
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Authors: | E B Konrad P Modrich I R Lehman |
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Institution: | Department of Biochemistry Stanford University School of Medicine Stanford, Calif. 94305, U.S.A. |
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Abstract: | ts7 an Escherichia coli 15 strain with a thermolabile DNA ligase, has previously been shown to be a temperature-sensitive conditional lethal mutant that is sensitive to methyl methane sulfonate and to ultraviolet irradiation; it also accumulates 10 S DNA fragments to an abnormal extent. When the ligase mutation is transferred to a wild-type E. coli K12 strain, the strain becomes temperature sensitive for growth and displays the same characteristics as ts7. These findings show that a functional DNA ligase is essential for normal DNA replication and repair in E. coli. |
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