Carboxyfluorescein diacetate succinimidyl ester fluorescent dye for cell labeling

Our objective was to study the properties of the carboxyfluorescein diacetate succinimidyl ester(CFDA-SE)and the methodology of cell labeling using CFDA-SE fluorescent dye.First,we analyzed the kinetics of CFDA-SE fluorescent dye intensity over time.Second,we determined the optimal concentration of CFDA-SE fluorescent dye for cell labeling.Third,we tested the toxicity of CFDA-SE fluorescent dye on labeled cells.Finally,we determined the optimal staining time of CFDA-SE fluorescent dye for cell labeling. The results show that the optimal concentration of CFDA-SE fluorescent dye for cell labeling varies according to different cell types.CFDA-SE fluorescent dye is non-toxic to cells as the cell death rate caused by CFDA- SE labeling is below 5%.The optimal cell labeling time was determined to be 8 min of incubation with CFDA-SE fluorescent dye.We concluded that the advantages of using CFDA-SE fluorescent dye for cell labeling are as follows:(1)the binding of CFDA-SE fluorescent dye to cells is stable;(2) CFDA-SE fluorescent dye is not toxic and does not modify the viability of labeled cells;and(3)CFDA-SE fluorescent dye is a suitable fluorochrome for cell labeling.