Characterisation of a monoclonal antibody against the fimbrial F8 antigen of Escherichia coli |
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| Authors: | Irmgard Moser Ida Ørskov Jörg Hacker Klaus Jann |
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| Institution: | Max-Planck-Institut für Immunbiologie, Stübeweg 51, D-7800 Freiburg, F.R.G.;Collaborative Centre for Reference and Research on Escherichia coli (World Health Organization), Statens Seruminstitut, DK-2300 Copenhagen, Denmark;Institut für Genetik und Mikrobiologie, Universität Würzburg, D-8700 Würzburg, F.R.G. |
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| Abstract: | Abstract A monoclonal IgG1 antibody against F8 fimbriae was obtained with the hybridoma technique using spleen cells from C3H/f mice immunised with a fimbrial preparation of Escherichia coli 2980 (O18ac:K5:H?:F1C, F8) and Sp 2/0 Ag8 myeloma cells. The hybrid cells were cloned twice by limiting dilution and grown in tissue culture. The monoclonal antibody was purified from culture supernatants on Protein A Sepharose. It reacted with F8 fimbriae in colony blot, enzyme-linked immunosorbent assay (ELISA) and immunoblot after electrotransfer from sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of fimbrial preparations. The antibody bound to and agglutinated F8-fimbriated bacteria. |
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| Keywords: | Fimbriae monoclonal antibody immunoblot ELISA mice |
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