Cytoplasmic protein vaccine against bacterial hemorrhagic septicemia (Aeromonosis) of fish |
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Authors: | L. P. Smirnov V. V. Bogdan N. N. Nemova L. N. Yukhimenko |
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Affiliation: | (1) Present address: Institute of Biology, Karelian Scientific Center, Russian Academy of Sciences, Pushkinskaya ul.11, 185610 Petrozavodsk, Karelia, Russia;(2) Present address: All-Russia Institute of Pond Fisheries, Rybnoe, Dmitrov district, 141821 Moscow oblast, Russia |
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Abstract: | Water-soluble proteins fromAeromonas sobria, a causative agent of bacterial hemorrhagic septicemia of fish, were separated into six fractions by gel chromatography on Sephadex G-100. Injections of fraction II (67 kDa) provided the highest protection of carp against the disease. Injections of proteins contained in fraction II caused stronger effects on certain biochemical parameters in the fish liver (fatty acids of phospholipids and cathepsin B and D activities) in comparison to infections of the live culture. |
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