Activation of alkyldihydroxyacetone phosphate synthase by detergents. |
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Authors: | C O Rock V Fitzgerald F Snyder |
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Affiliation: | Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030 USA |
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Abstract: | The protein pairs S3-S4, S4-S5, and S4-S20 isolated from the 30 S subunit of the Escherichia coli ribosome were investigated by the method of sedimentation equilibrium for possible intermolecular interactions in the reconstitution buffer. The pairs S3-S4 and S4-S5 were found to interact in TMK buffer (0.03 m Tris, 0.02 m MgCl2, 0.35 m KCl, pH 7.4) at 4 °C with free energies of interaction of ?5.1 and ?4.8 kcal/mol, respectively. The pair S4-S20 demonstrated no tendency to complex under the same conditions. |
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