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Fluorescent labeling of ursolic acid with FITC for investigation of its cytotoxic activity using confocal microscopy |
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| Institution: | 1. Federal Research Center Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences, 10, Lavrentyev Ave., 630090 Novosibirsk, Russia;2. Novosibirsk Institute of Organic Chemistry of Siberian Branch of the Russian Academy of Sciences, 10, Lavrentyev Ave., 630090 Novosibirsk, Russia;3. Novosibirsk State University, 2, Pirogov Street, 630090 Novosibirsk, Russia;4. Federal Research Center of Fundamental and Translational Medicine of Siberian Branch of the Russian Academy of Sciences, 2, Timakov Street, 630117 Novosibirsk, Russia;1. Institute of Medicinal Biotechnology, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100050, China;2. School of Pharmacy, Jinzhou Medical University, Jinzhou, Liaoning 121001, China;1. Department of Human Anatomy, School of Medicine, Jinan University, Guangzhou 510632, China;2. College of Materials and Energy, South China Agricultural University, Guangzhou 510642, China;3. Department of Ophthalmology, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China;4. School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China;1. University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh;2. Institut für Pharmakologie und Toxikologie, Universität Würzburg, Germany;1. Departamento de Química Orgánica, Facultad de Ciencias, Universidad de Granada, E-18071, Granada, Spain;2. Departamento de Bioquímica y Biología Molecular I, Facultad de Ciencias, Universidad de Granada, E-18071, Granada, Spain |
| Abstract: | Fluorescent labeling is a widely-used approach in the study of intracellular processes. This method is becoming increasingly popular for studying small bioactive molecules of natural origin; it allows us to estimate the vital intracellular changes which occur under their influence. We propose a new approach for visualization of the intracellular distribution of triterpene acids, based on fluorescent labeling by fluoresceine isothiocyanate. As a model compound we took the most widely-used and best-studied acid in the ursane series – ursolic acid, as this enabled us to compare the results obtained during our research with the available data, in order to evaluate the validity of the proposed method. Experimental tracing of the dynamics of penetration and distribution of the labeled ursolic acid has shown that when the acid enters the cell, it initially localizes on the inner membranes where the predicted target Akt1/protein kinase B – a protein that inhibits apoptosis – is located. |
| Keywords: | Triterpene acids Ursolic acid Fluorescence Cytotoxicity Akt1/protein kinase B MDM2/E3 ubiquitin-protein ligase DMSO"} {"#name":"keyword" "$":{"id":"k0040"} "$$":[{"#name":"text" "_":"dimethyl sulfoxide FITC"} {"#name":"keyword" "$":{"id":"k0050"} "$$":[{"#name":"text" "_":"fluoresceine isothiocyanate UA"} {"#name":"keyword" "$":{"id":"k0060"} "$$":[{"#name":"text" "_":"ursolic acid beta-epimer of fluorescent ladeled ursolic acid MD"} {"#name":"keyword" "$":{"id":"k0080"} "$$":[{"#name":"text" "_":"molecular dynamics |
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