A preliminary study on the use of jenny colostrum to improve quality in extenders for freezing donkey semen |
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| Institution: | 1. Horse Breeding Military Center of Zaragoza, 50190, Zaragoza, Spain;2. Department of Animal Pathology, Faculty of Veterinary Medicine, Instituto Agroalimentario de Aragón IA2 (Universidad de Zaragoza-CITA), Zaragoza, Spain;1. Veterinary Reproduction Group, Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, University of Cordoba, 14071 Cordoba, Spain;2. Equine Reproduction Center, Centro de Selección y Reproducción Animal (CENSYRA-Extremadura Government), 06007 Badajoz, Spain;3. Department of Biology, Genetics Unit, Autonomous University of Madrid, 20849 Madrid, Spain;1. Veterinary Reproduction Group, University of Cordoba, Cordoba, Spain;2. Equine Reproduction Center, Centro de Selección y Reproducción Animal, (CENSYRA-Extremadura Government), Badajoz, Spain;1. Veterinary Reproduction Group, Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, University of Cordoba, 14071 Cordoba, Spain;2. IGEVET – Instituto de Genética Veterinaria \"Ing. Fernando N. Dulout\" (UNLP - CONICET LA PLATA), 1900 La Plata, Argentina;3. Department of Animal Production. Faculty of Veterinary Sciences. National University of La Plata, 1900 La Plata, Argentina;4. Equine Reproduction Center, Centro de Selección y Reproducción Animal (CENSYRA-Extremadura Government), 06007 Badajoz, Spain;5. Department of Genetics, Faculty of Veterinary Medicine, University of Cordoba, 14071 Cordoba, Spain;1. Veterinary Reproduction Group, Department of Animal Medicine Surgery, University of Cordoba, 14071 Cordoba, Spain;2. Department of Veterinary Medical Sciences, Bologna University, 40126 Bologna, Italy;3. National Institute for Artificial Insemination, University of Bologna, 40126 Bologna, Italy;4. Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Texas A&M University, College Station, TX 77843, United States;1. Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Veterinary Reproduction Group, University of Cordoba, Cordoba, Spain;2. Departamento de Producción Animal, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, Buenos Aires, Argentina;3. Department of Genetics, Faculty of Veterinary Medicine, MERAGEM Group, University of Cordoba, Cordoba, Spain |
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| Abstract: | Sperm quality in donkeys (Equus asinus) after freezing thawing is still considered lower than that from other animals, including horses. The aim of this study was to test a new freezing extender supplemented with jenny colostrum on donkey sperm. After thawing, we evaluated sperm motility by means of computer-assisted analysis, viability by SYBR-14 and propidium iodide (PI), membrane functional integrity by HOS-test and acrosome integrity by isothiocyanate conjugated with peanut agglutinin (FITC-PNA) and PI. Ejaculates were collected from five fertile Donkeys. Sperm samples were pooled, diluted and cryopreserved into three experimental extender groups: BotuCrio®, lactose-extender supplemented with egg yolk (20%) and lactose-extender supplemented with jenny colostrum (20%). The results demonstrated that lactose-jenny colostrum samples displayed significantly higher values in almost all parameters evaluated (p < 0.05) compared with the other two extenders after thawing (BotuCrio® and lactose-egg yolk based extender, respectively) –Total Motility, Viability, HOS test, VCL, VSL and VAP. Acrosome status, LIN, STR and WOB despite showing lower values, none of them were statistically significant (p > 0.05). In conclusion, the extender containing jenny colostrum can be successfully used for donkey semen crypreservation and could effectively improve donkey sperm qualities after freezing -thawing. |
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| Keywords: | Jenny Colostrum Donkey Semen Cryopreservation |
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