Pre-conditioning with Xanthine oxidase to improve post thawed quality of bull sperm |
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| Affiliation: | 1. Veterinary Physiology, Vetsuisse Faculty, University of Bern, 3001 Bern, Switzerland;2. Department of Animal Science, Agriculture Faculty, Yasouj University, 75918-74831, Yasouj, I. R. Iran;1. College of Animal Science and Technology, Beijing University of Agriculture/Beijing, China;2. Department of Agricultural, Forest and Food Sciences, University of Torino/Grugliasco, Italy;1. Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran;1. Department of Animal Science, University of Tabriz, Tabriz, Iran;2. Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran;3. Department of Animal Science, College of Agriculture, Tarbiat Modarres University, Iran;1. Department of Agricultural, Environmental, and Food Sciences, University of Molise, Campobasso, Italy;2. Department of Medicine and Health Sciences “Vincenzo Tiberio”, University of Molise, Campobasso, Italy;3. Département des Sciences Animales, Centre de Recherche en Biologie de la Reproduction, Université Laval, Québec, Canada;1. Animal Science Research Institute of Iran (ASRI), Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran;2. Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran |
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| Abstract: | The purpose of this study was to examine the effects of sub-lethal concentration of Xanthine oxidase (XO) on the post-thawed bull sperm quality. Semen samples were collected from four Holstein bulls, twice a week and during three consecutive weeks (n = 24 total ejaculates). After collection in each replicate, semen samples were pooled and then frozen by semen extender containing different concentrations [0 (XO-0), 0.05 (XO-0.05), 0.5 (XO-0.5), 5 (XO-5), 50 (XO-50) and 500 (XO-500) μM] of XO. After thawing, motion parameters (SCA), plasma membrane functionality (HOST), apoptosis status (Phosphatidylserine translocation assay), mitochondrial activity (Rhodamine 123), and acrosome integrity (PSA), were evaluated. The results showed that total motility, VAP, VSL, VCL, STR, and LIN were lower in XO-50 and XO-500 compared to other groups (P < 0.05). Progressive motility were higher in XO-0.05 and XO-0.5 compared to XO-0, XO-50, and XO-500 (P < 0.05). Mitochondrial activity was highest in XO-0.05 and XO-0.5 groups. Sperm plasma membrane functionality was significantly greater in XO-0, XO-0.05, XO-0.5, and XO-5 than that of XO-50 and XO-500. Xanthine oxidase had not significant effects on acrosome integrity and dead spermatozoa. Higher percentage of live spermatozoa was recorded for XO-0, XO-0.05, XO-0.5, and XO-5; however, the lower amount of apoptotic spermatozoa was detected in the aforementioned groups (P < 0.05). In conclusion, it seems that XO at lower doses may have beneficial effects on post-thawed bull sperm quality. |
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| Keywords: | Bull Freezing Semen Stress |
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