Changes of pollen viability of ornamental plants after long-term preservation in a cryopreservation pollen bank |
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| Institution: | 1. Beijing Laboratory of Urban and Rural Ecological Environment, Beijing Municipal Education Commission, Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, College of Landscape Architecture, Beijing Forestry University, Beijing, 100083, China;2. College of Architecture and Urban Planning, Guangzhou University, Guangzhou, 510006, China;3. Luoyang Aerospace Peony Garden Co. Ltd, Henan, Luoyang, 471000, China;1. Institute of Zoology, Slovak Academy of Sciences, Dúbravska cesta 9, 845 06, Bratislava, Slovakia;2. Department of Ecosystem Biology, Faculty of Science, University of South Bohemia, Branišovská 31, České Budějovice, 37005, Czech Republic;3. Department of Biology, Faculty of Education, Priemyselná 4, 918 43, Trnava, Slovakia;4. Department of Chemistry, Faculty of Education, Priemyselná 4, 918 43, Trnava, Slovakia;1. Plant and Animal Genetic Resources Preservation Unit, National Laboratory for Genetic Resources Preservation, USDA-ARS, Fort Collins, CO 80521, USA;2. Plains Area, USDA-ARS, Fort Collins, CO 80521, USA;3. National Clonal Repository, USDA-ARS, Corvallis, OR 97333, USA;1. Institute of Systems Biology, Universiti Kebangsaan Malaysia, 43600 UKM, Bangi, Selangor, Malaysia;2. Retired, US Dept. of Agriculture and Department of Horticulture, Oregon State University, Corvallis, OR, 97331, USA |
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| Abstract: | This study determined the changes in pollen viability of 102 species/cultivars of ornamental plants (affiliated to 32 genera of 14 families) following long-term liquid nitrogen storage in a cryopreservation pollen bank. The goal was to provide information on the safety and stability of pollen cryopreservation technology. Fresh pollen at the time of storage was used as the control, and the study examined the pollen viability of ornamental plants cryopreserved for 8, 9, or 10 years. The results show that pollen of the 102 species/cultivars in the cryopreservation pollen bank retained viability ranging from 1% to 58%, After long-term storage there were changes in viability: 11.76% (12 species/cultivars) had increased viability, 16.67% (17 species/cultivars) had stable viability, and the viability of 71.57% (73 species/cultivars) showed a decreasing trend. |
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| Keywords: | Cryopreservation Long-term storage Ornamental plants Pollen bank Viability |
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