Cryopreservation disrupts lipid rafts and heat shock proteins in yellow catfish sperm |
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| Institution: | 1. The Second Affiliated Hospital and Yuying Children''s Hospital, Wenzhou Medical University, Wenzhou 325035, PR China;2. Institute of Environmental Safety and Human Health, Wenzhou Medical University, Wenzhou 325035, PR China;1. Laboratory of Aquaculture, Federal University of Rio Grande do Sul, 7712, Bento Gonçalves Avenue, Agronomia, Porto Alegre 91540-000, RS, Brazil;2. Laboratory of Biotechnology Applied to Fish Reproduction, Nilton Lins University, 3259, Professor Nilton Lins Avenue, Parque das Laranjeiras, Manaus 69058-030, AM, Brazil;3. INRA, UR1037, Fish Physiology and Genomics, Campus de Beaulieu, 35000 Rennes, France;1. Department of Experimental Medicine, University of Genoa, Genova, Italy;2. Department of Biomedical Sciences for Health, Università Degli Studi di Milano, Milan, Italy;3. Metabolism Research Center, Endocrinology and Metabolism, IRCCS Policlinico San Donato, San Donato Milanese, Italy;4. IRCCS Istituto Ortopedico Galeazzi, Milan, Italy;1. Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, China;2. Center for Reproductive Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University; Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Shanghai 200135, China;3. Maternal and Child Health Hospital of Anhui Province, The Maternal and Child Health Clinical College, Anhui Medical University, Hefei, China |
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| Abstract: | Lipid rafts and associated membrane proteins (flotillin, caveolin) play important roles in cell signaling and sperm fertilization while heat shock proteins (Hsp) ensure properly protein folding to fulfill their physiological functions. The markedly reduced fertility in thawed sperm after cryopreservation could result from disrupted membrane lipid rafts and these proteins. To explore the effect of sperm cryopreservation on lipid rafts and heat shock proteins, we compared lipid raft integrity, and the expression levels of lipid raft associated proteins (Flot-1, Flot-2, Cav-1) as well as heat shock proteins (Hsp90, Hsp70) in fresh and thawed sperm cryopreserved under different scenarios in yellow catfish. We found higher lipid raft integrity, higher protein expression levels of Flot-1, Flot-2, Cav-1, Hsp90, and Hsp70 in fresh sperm samples than in thawed sperm samples, in thawed sperm samples cryopreserved with optimal cooling rate than those cryopreserved with sub-optimal cooling rate, and in thawed sperm samples cryopreserved with extenders supplemented with cholesterol than those supplemented with methyl-β-cyclodextrin (for cholesterol removal). Our findings indicate that lipid raft integrity, and expression levels of Flot-1, Flot-2, Cav-1, Hsp90, and Hsp70 are clearly associated with sperm quality, and together they may play a cumulative role in reduced fertility associated with thawed sperm in aquatic species. |
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| Keywords: | Sperm cryopreservation Lipid raft Lipid raft protein Heat shock protein Cholesterol |
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