Cryopreservation and hormonal induction of spermic urine in a novel species: The smooth-sided toad (Rhaebo guttatus) |
| |
| Institution: | 1. Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia;2. Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia;3. Sustainability America, La Isla Road, Sarteneja, Corozal District, Belize;1. Department of Zoology, Savitribai Phule Pune University, Pune 411007, India;2. School of Science and Health, Western Sydney University, Locked Bag 1797, Penrith, NSW 2751, Australia;1. Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Biodiversidad y Biología Experimental, Buenos Aires, Argentina;2. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina;3. Instituto de Biodiversidad y Biología Experimental y Aplicada (IBBEA – CONICET), Buenos Aires, Argentina;4. Instituto de Diversidad y Evolución Austral (IDEAus – CONICET), Puerto Madryn, Chubut, Argentina;1. Programa de Pós-Graduação em Biodiversidade, Universidade Federal do Oeste do Pará, Rua Vera Paz, Santarém, Pará, Brazil;2. Instituto Neotropical: Pesquisa e Conservação, Curitiba, Paraná, Brazil;1. Programa de Pós-Graduação em Biodiversidade Neotropical, Universidade Federal da Integração Latino Americana, Foz do Iguaçu, Paraná, Brazil;2. Programa de Pós-Graduação em Conservação e Manejo de Recursos Naturais, Universidade Estadual do Oeste do Paraná, Cascavel, Paraná, Brazil;1. Programa de Pós-Graduação em Biodiversidade, Universidade Federal do Oeste do Pará, Rua Vera Paz, Santarém, Pará, Brazil;2. Laboratório de Ecologia e Conservação, Instituto de Biodiversidade e Florestas, Universidade Federal do Oeste do Pará, Brazil;1. Biology Department, Faculty of Sciences, Shiraz University, Shiraz, Iran;2. Shiraz University of Medical Sciences, Shiraz, Iran;1. Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Starkville, Mississippi, USA;2. Department of Conservation and Research, Memphis Zoo, Memphis, Tennessee, USA;3. Colorado Department of Parks and Wildlife, Native Aquatic Species Restoration Facility, Alamosa, Colorado, USA |
| |
| Abstract: | Global amphibian declines have fueled an increased interest in amphibian assisted reproductive technologies. Within the genus Rhaebo, half of the species are experiencing decreasing population trends; however, insufficient information is available on many of these species’ reproductive biology. Using the smooth-sided toad, Rhaebo guttatus, we present effective methods for collecting and cryopreserving an example of Rhaebo sperm. Specifically, our findings show that administering 10 IU/g body weight of hCG (human chorionic gonadotropin) yields the most motile and concentrated sperm and that cryopreserving spermic urine in a solution of 5% DMFA (N,N-Dimethylformamide) and 10% trehalose returns sperm with a 33 ± 3% average post-thaw motility. These findings may represent an important step forward in developing techniques that can be safely applied to other, more vulnerable species within the Rhaebo genus. |
| |
| Keywords: | Amphibian Assisted reproductive technologies Anura Spermatozoa Bufonidae Human chorionic gonadotropin |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
