Cryopreservation of cell line Paesun by a rapid cooling |
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| Institution: | 1. Department of Paleontology, Faculty of Geology, Kim Il Sung University, Pyongyang, Democratic People''s Republic of Korea;2. State Key Laboratory of Palaeobiology and Stratigraphy, Nanjing Institute of Geology and Palaeontology and Center for Excellence in Life and Paleoenvironment, Chinese Academy of Sciences, Nanjing, 210008, China;3. University of Science and Technology of China, Hefei, 230026, China;1. Faculty of Pharmacy, Pyongyang Medical College, Kim Il Sung University, Ryomyong Str., Pyongyang, Democratic People’s Republic of Korea;2. Institute of Biochemistry, University Medicine Berlin-Charité, Chariteplatz 1, D-10117, Berlin, Germany;1. Charité – Universitätsmedizin Berlin, Department of Dermatology, Venerology and Allergology, Center of Experimental and Applied Cutaneous Physiology, Charitéplatz 1, 10117 Berlin, Germany;2. Kim Il Sung University, Ryongnam-Dong, Taesong District, Pyongyang, Democratic People''s Republic of Korea |
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| Abstract: | The purpose of the present study was to clarify the possibility of a rapid cryopreservation for cell line Paesun by cooling in the range of 30–40 °C/min to vapor phase of ?120 ～-140 °C before immersion into liquid phase of liquid nitrogen using 10% Me2SO. After thawing, these cells were examined with assaying viability by trypan blue exclusion staining and survival by cloning in monolayer; the percentages of cell and colony recovery obtained in rapid cooling had a tendency to be lower than that by slow cooling of 1 °C/min but there were no significant differences between them. In addition, post-thaw cells were examined by assaying proliferation and susceptibility to virus lines; there were no significant differences between before and after cryopreservation. In conclusion, these findings indicate that Paesun can be successfully cryopreserved by the rapid cooling rate of 30 °C–40 °C/min. |
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| Keywords: | Cryopreservation Cell line Paesun Rapid cooling |
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