Production of F0 mice from embryonic stem cells injected eight-cell stage embryos which stored at refrigeration temperature |
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| Affiliation: | 1. Chongqing Academy of Animal Sciences, Chongqing, 402460, China;2. Key Laboratory of Pig Industry Sciences, Ministry of Agriculture, Chongqing, 402460, China;3. Key Laboratory of Pig Industry Sciences, Chongqing, 402460, China;1. Jilin Province Key Laboratory of Animal Embryo Engineering, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, Jilin 130062, China;2. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China;1. Department of Psychology, Northeastern University, United States;2. University of North Carolina, United States;3. Kinsey Institute, Indiana University, United States;1. Department of Genetic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon, South Korea;2. Department of East-West Medical Science, Graduate School of East-West Medical Science, Kyung Hee University, Yongin 446-701, South Korea;3. Division of Radiation Cancer Research, Korea Institute of Radiological and Medical Sciences, Seoul, South Korea;1. Fundamental and Applied Cryobiology Group, Department of Biochemistry and Cellular and Molecular Biology, The University of Tennessee, Knoxville, TN 37996, USA;2. Present address: Tokyo University of Marine Science and Technology, Tokyo 108-8477, Japan |
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| Abstract: | At refrigeration temperature, mouse embryos can retain their developmental ability for a couple of days. Previous research reports have focused on the effect of cool temperature on the development of 2-cell stage embryos, morulae or blastocysts and determined that the embryo still has the ability to produce offspring after about 48 h storage at refrigeration temperature. Here we examined whether refrigeration temperature affects the development of the eight-cell stage and if the stored eight-cell stage embryo can still be used as a host embryo for ES cell injection. Our results show that eight-cell stage embryos can develop into blastocysts and yield pups after cold storage for 24 and 48 h. After ES cell injection, stored eight-cell stage embryos can support ES cells developing to F0 pups. In summary, cool storage can preserve the developmental ability of eight-cell stage embryos for at least 48 h, allowing transportation of the embryos at refrigeration temperature between different labs and their subsequent use as host embryos for ES cell injection. |
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| Keywords: | Eight-cell stage embryo Refrigeration temperature ES cell injection F0 mouse |
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