Nylon mesh cryodevice for bovine mature oocytes,easily removable excess vitrification solution |
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| Affiliation: | 1. Shanghai Ji Ai Genetics & IVF Institute, Obstetrics & Gynecology Hospital, Fudan University, Shanghai, 200011, China;2. Reproductive Medical Center, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200025, China;1. Laboratorio de Biotecnología Animal, Facultad de Agronomía, Universidad de Buenos Aires/INPA-CONICET, Av. San Martín 4453, P.C. 1417, Buenos Aires, Argentina;2. Laboratorio de Reproducción, Centro de Biotecnología de la Reproducción (CEBIOR-BIOREN), Universidad de La Frontera, Montevideo 0870, Temuco, Chile;1. Department of Andrology, People’s Hospital of Liaocheng City, China;2. Department of Obstetrics and Gynecology, People’s Hospital of Linyi City, China;3. Department of Reproductive Medicine, Jinan Central Hospital Affiliated to Shandong University, China;4. Department of Reproductive Medicine, Hospital for Maternity and Child Care of Jinan City, China;1. Laboratory on Animal Germplasm Conservation, Universidade Federal Rural Do Semi-Árido (UFERSA), BR 110, Km 47, Costa e Silva, 59625-900, Mossoró, RN, Brazil;2. Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculdade de Veterinária, Universidade Do Ceara, Av. Paranjana 1700, Campus Do Itaperi, 60714-903, Fortaleza, CE, Brazil;3. Laboratory of Animal Biotechnology, UFERSA, BR 110, Km 47, Costa e Silva, 59625-900, Mossoró, RN, Brazil;1. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche, Área de Producción Animal, Laboratorio de Reproducción de Rumiantes Menores. Instituto de Investigaciones Forestales y Agropecuarias Bariloche (INTA-CONICET), Bariloche, Argentina;2. Instituto de Biología y Medicina Experimental (CONICET), Laboratorio de Regulación Hipofisaria, Buenos Aires, Argentina |
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| Abstract: | The aim of this study was to determine pore size of nylon mesh (NM) device suitable for cryosurvival of bovine mature oocytes and to apply the device to vitrification of large quantities of the oocytes. Ten to twelve oocytes were loaded onto an NM device (a square opening 37-, 57- or 77-μm on a side length). After removal of the excess volume of vitrification solution by paper absorption, the oocytes were vitrified-warmed, fertilized and cultured in vitro. Oocyte recovery and morphological survival were comparable among the three groups. However, blastocyst yield in the 37-μm group (39%) was higher than that in the 77-μm group (28%), and the yield in the 57-μm group (31%) was the intermediate. The 37-μm NM device was applicable for increased oocyte number >40 (blastocyst yield, 33%). These results suggest that 37-μm-pore sized NM can serve as cryodevice to vitrify large quantities of in vitro-matured bovine oocytes. |
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| Keywords: | Bovine oocytes Cooling-warming rate IVM-IVF-IVC Large sample scale Mesh size Pipet operation-free Vitrification-warming |
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