Dimethyl sulfoxide maintains structure and function of cryopreserved equine endometrial explants |
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| Affiliation: | 1. Department of Animal Reproduction and Veterinary Radiology, Universidade Estadual Paulista (UNESP), São Paulo, Brazil;2. Department of Theriogenology, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana, USA;3. Department of Veterinary Clinics, Universidade Estadual Paulista (UNESP), São Paulo, Brazil;1. Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA, 70803, USA;2. Faculty of Veterinary Medicine, University of Calgary, Calgary, AB, T2N 4Z6, Canada;3. Department of Veterinary Biosciences, College of Veterinary Medicine, Ohio State University, Columbus, OH, 43210, USA;1. Departamento de Reprodução e Avaliação Animal, Universidade Federal Rural Do Rio de Janeiro, Seropédica, Rio de Janeiro, 23897-000, Brazil;2. Eutheria Foundation, Cross Plains, WI, 53528, USA;3. Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, 53706, USA;1. Institute for Animal Breeding and Genetics, University of Veterinary Medicine Hannover (Foundation), Hannover, Germany;2. Unit of Reproductive Medicine—Clinic for Horses, University of Veterinary Medicine Hannover (Foundation), Hannover, Germany;3. Lower Saxon National Stud Celle, Celle, Germany;1. Department of Clinical Sciences, Cornell University, 930 Campus Rd, Ithaca, NY, 14853, USA;2. Department of Veterinary Clinical and Diagnostic Science, University of Calgary, 2500 University Drive NW, Calgary, Alberta, T2N 1N4, Canada;3. Department of Large Animal Clinical Sciences, Western College of Veterinary Medicine, 52 Campus Drive, Saskatoon, SK, S7N 5B4, Canada |
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| Abstract: | Availability of viable frozen-thawed endometrial tissues could facilitate detailed studies into physiologic and disease processes influencing the endometrium. This study was designed to investigate the cryosurvival of equine endometrial tissue. Previous studies in the human and horse have focused on cryopreservation of dissociated endometrial cells. To our knowledge, there are no studies on cryopreservation of endometrial explants. Our objectives were to 1) determine the influence of differing concentrations of the permeating cryoprotectant dimethyl sulfoxide (Me2SO) on viability, structural integrity, and gene expression of cryopreserved equine endometrial tissues prior to and following a 5-day explant culture in vitro and 2) examine the influence of low (1000 mg/L dextrose) vs high (4500 mg/L dextrose) glucose medium during in vitro culture. Both 10% and 20% (v/v) concentrations of Me2SO maintained viability following cryopreservation and in vitro culture. In addition, gene expression remained unaltered following cryopreservation with either 10% or 20% Me2SO. However, tissue structural integrity was slightly reduced compared to the fresh control. Furthermore, there was no difference in structural integrity, cell viability, or gene expression between low and high glucose medium during in vitro culture. Although E-cadherin and Ki67 gene expression was not different among fresh, 10% Me2SO, and 20% Me2SO treatments prior to or following tissue culture, estrogen receptor-α and progesterone receptor gene expression were reduced in all groups after explant culture. This is the first report of successful cryopreservation of equine endometrial explants. |
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| Keywords: | Cryopreservation Equine Endometrium Explant Mare Dimethyl sulfoxide |
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