Antioxidant and angiotensin-converting enzyme (ACE) inhibitory activity of thymosin alpha-1 (Thα1) peptide |
| |
| Institution: | 1. Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran;2. Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran;1. State Key Laboratory of Bioreactor Engineering, Department of Food Science and Technology, School of Biotechnology, East China University of Science and Technology, Shanghai 200237, PR China;2. Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei Key Laboratory of Industrial Microbiology, Hubei University of Technology, Wuhan 430068, PR China;3. Sports Health Center, Tongji University, Shanghai 200092, PR China;4. Shanghai Collaborative Innovation Center for Biomanufacturing (SCICB), Shanghai 200237, PR China;1. Marine-Integrated Bionics Research Center, Pukyong National University, Busan 48513, Republic of Korea;2. Department of Biomedical Engineering, and Center for Marine-Integrated BiomedicalTechnology (BK21 Plus) Pukyong National University, Busan 48513, Republic of Korea;3. Environmental Safety Group, Korea Institute of Science and Technology Europe (KIST Europe), Campus E 7.1, 66123 Saarbruecken, Germany;4. Jeju International Marine Science Center for Research & Education, Korea Institute of Ocean Science and Technology, Jeju 63349, Republic of Korea;5. Department of Microbiology, College of Medicine, Inje University, Busan 47392, Republic of Korea;6. Department of Physiology, Kangwon National University School of Medicine, Chuncheon 24341, Republic of Korea;7. Department of Marine Biology, University of Science and Technology, Daejeon 34113, Republic of Korea;1. Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB T6G 2P5, Canada;2. College of Food Engineering, Central South University of Forestry and Technology, Changsha 410004, PR China;1. School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu Province 212018, PR China;2. Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang, Jiangsu Province 212018, PR China |
| |
| Abstract: | In this research, the antioxidant property of thymosin alpha-1 (Thα1) peptide was investigated through various antioxidant methods. Thα1 showed 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (IC50 = 20 µM) and its 2,2-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) scavenging reached 45.33% at 80 µM (IC50 = 85 µM). In addition, hydroxyl and superoxide radical scavenging of Thα1 peptide exhibited a concentration-depended manner. The IC50 values of hydroxyl and superoxide radical scavenging were estimated to be 82 µM and 20 µM, respectively. The effect of Thα1 on eliminating superoxide radicals was higher (62.23%) than other antioxidant assays. Moreover, the antioxidant activity of Thα1 peptide was evaluated by measuring cellular reactive oxygen species (ROS). Results indicated that Thα1 decreased the generation of ROS level in 1321 N1 human neural asterocytoma cells. The inhibitory effect of Thα1 on angiotensin-converting enzyme (ACE) was determined. The kinetic parameters (Km and Vmax) and the inhibition pattern were examined. Based on the Lineweaver-Burk plot, Thα1 displayed a mixed inhibition pattern. The IC50 and Ki values of Thα1 were 0.8 µM and 3.33 µM, respectively. Molecular modeling suggested that Thα1 binds to ACE-domains with higher affinity binding to N-domain with the binding energy of −22.87 kcal/mol. Molecular docking indicated that Thα1 interacted with ACE enzyme (N- and C-domains) due to electrostatic, hydrophobic, and hydrogen forces. Our findings suggested that Thα1 possess a multifunctional peptide with dual antioxidant and ACE-inhibitory properties. Further researches are needed to investigate the antioxidant and anti-hypertensive effect of Thα1 both in vitro and in vivo. |
| |
| Keywords: | Natural peptides ACE Thymosin alpha-1 Antioxidant assays Molecular docking |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
