Antioxidative effect of melatonin on cryopreserved chicken semen |
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| Institution: | 1. Departamento de Ciencias Agroforestales, Universidad de Huelva, “Campus de Excelencia Internacional Agroalimentario, ceiA3”, Carretera Huelva-Palos de la Frontera s/n, 21819 Palos de la Frontera, Huelva, Spain;2. Universidad Arturo Prat, Facultad de Recursos Naturales Renovables, Avenida Arturo Prat, 2120 Iquique, Chile;3. Departamento de Reproducción Animal, SGIT-INIA, Madrid, Spain;1. Animal Science Research Institute of Iran (ASRI), Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran;2. Department of Poultry Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran;1. Department of Physiology and Radiobiology, Faculty of Veterinary Medicine, University of Zagreb, 10 000, Zagreb, Croatia;2. Clinic of Reproduction and Obstetrics, Faculty of Veterinary Medicine, University of Zagreb, 10 000, Zagreb, Croatia;3. Department of Pathophysiology, Faculty of Veterinary Medicine, University of Zagreb, 10 000, Zagreb, Croatia;4. Department of Animal Nutrition and Dietetics, Faculty of Veterinary Medicine, University of Zagreb, 10 000, Zagreb, Croatia;5. Veterinary Practice Vara?din, 42000, Vara?din, Croatia;6. Faculty of Veterinary Medicine, University of Zagreb, 10 000, Zagreb, Croatia;1. Department of Animal Science, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran;2. Department of Poultry Science, Faculty of Agriculture, University of Tarbiat Modares, Tehran, Iran;3. Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran;4. Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran;1. Department of Poultry Production, Faculty of Agriculture, Assiut University, 71526 Assiut, Egypt;2. Departamento de Reproducción Animal, Instituto Nacional de Investigación y Tecnologia Agraria y Alimentaria (INIA), 28040 Madrid, Spain |
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| Abstract: | This is a unique study because is the first time we are adding melatonin into an extender in order to determine its influence on cryopreserved chicken semen. The primary focus of our present study was to evaluate the influence of different concentrations of Melatonin on cryopreserved chicken semen. Semen samples were allocated into four treatments, being one control and three different combinations of antioxidants and after the freeze-thaw operation, the sperm motility, plasma membrane integrity, acrosome integrity, endogenous enzymes (GSH-Px, CAT, SOD), MDA and ROS of chicken spermatozoa were all evaluated. The collection of the semen samples was from 40 Arbor Acre roosters and this procedure was repeated twice a week and then mixed in an extender that contained different MEL treatments as follows: a diluent without MEL (control, M 0), a diluent comprising 0.125 mg/mL (M 0.125) 0.25 mg/mL, (M 0.25) and 0.5 mg/mL (M 0.5). It was revealed that the supplementation of the base extender with an optimal 0.25 mg/mL MEL led to a higher significant difference in the motility of chicken sperm (P < 0.01), higher acrosome integrity (P < 0.05) and a higher plasma membrane integrity (P < 0.01) when compared to the control group at post-thaw. Furthermore, when compared to the control group, 0.25 mg/mL MEL addition into the extender significantly enhanced the activity of endogenous enzymes (GSH-Px, CAT, and SOD) in the chicken spermatozoa at post-thaw (P < 0.05). Moreover, 0.5 mg/mL MEL supplementation into the extender enhanced the GSH-Px activity in the chicken spermatozoa when compared with the control group (P < 0.05) at post-thaw. In contrast, the addition of 0.25 mg/mL MEL into the extender resulted in a significantly lower MDA in comparison to the 0.125 mg/mL, 0.5 mg/mL MEL treatment group and the control group (P < 0.05). Also, compared to the control group, MEL concentration of 0.125 mg/mL and 0.5 mg/mL MEL into the extender resulted in a significantly low ROS concentration (P < 0.05) but the addition of 0.25 mg/mL MEL concentration resulted in a significantly lower ROS level when compared to the control group (P < 0.01). In summary, MEL improved the quality of cryopreserved chicken sperm quality by decreasing oxidative stress level and the most optimal concentration was 0.25 mg/mL. |
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| Keywords: | Melatonin Cryopreservation Chicken Spermatozoa |
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