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Uncoupling the hydrolysis of lipid-linked oligosaccharide from the oligosaccharyl transfer reaction by point mutations in yeast oligosaccharyltransferase |
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| Authors: | Takahiro Yamasaki Daisuke Kohda |
| Affiliation: | Division of Structural Biology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan |
| Abstract: | |
| Keywords: | endoplasmic reticulum (ER) epitope tag free N-glycan (FNG) glycoprotein biosynthesis membrane enzyme N-linked glycosylation oligosaccharyltransferase (OST) Saccharomyces cerevisiae site-directed mutagenesis substrate specificity oligosaccharyltransferase free oligosaccharide (fOS) |