An investigation into the genotoxic species generated during reduction of chromium(VI) by catechol(amine)s |
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Abstract: | AbstractChromium(VI) is a common occupational carcinogen.1 The major carcinogenic and mutagenic species are proposed to be Cr(V) and Cr(IV) intermediates formed during the reduction of Cr(VI) to stable Cr(III) compounds,2 although indirect evidence suggests that reactive oxygen species (ROS) may also be important.3 The reductions of Cr(VI) by some biological reductants (e.g. ascorbate) have been studied previously, and genotoxic Cr(IV/V) species have been detected.4 Another potential reductant in vivo is protein-bound DOPA, which is present on oxidised proteins at low steady-state concentrations prior to enzymatic breakdown.5 Recently, we have shown, by EPR spectroscopy, that the reactions of Cr(VI) with model DOPA compounds (catechol(amine)s), and with oxidised proteins themselves, generate several reactive intermediates, including Cr(V) complexes and organic radicals.6 Previous studies have proposed that ROS may also be produced during catechol(amine) oxidation.7 Here we describe studies of the interaction of DNA with the reactive species produced during the reductions of K2Cr2O7 by catechol(amine)s. |
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