Physical Properties of Lactic Dehydrogenase-Elevating Virus and Its Ribonucleic Acid |
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Authors: | Margo B Darnell and Peter G W Plagemann |
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Institution: | Department of Microbiology, The University of Minnesota Medical School, Minneapolis, Minnesota 55455 |
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Abstract: | Infectious lactic dehydrogenase-elevating virus propagated in primary cultures of mouse peritoneal macrophages in the presence of 3H-uridine and isolated by isopycnic centrifugation was found to have a density of 1.12 g/cm3. Ribonucleic acid extracted from the virus by treatment with sodium dodecyl sulfate was single stranded with a sedimentation coefficient of approximately 48S. |
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