Significance of O2 availability and cycling on the respiratory burst response of human PMN's exposed to cytochrome c and superoxide dismutase |
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Authors: | T R Green R E Schaefer M T Makler |
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Affiliation: | 1. Veterans Medical Center Health Sciences Center Portland, Oregon 97201 USA;2. University of Oregon Health Sciences Center Portland, Oregon 97201 USA |
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Abstract: | In many instances the effect of superoxide () trapping agents in suppressing the net rate of O2 consumption of activated PMN's is not in accordance with theoretical expectations. We offer here an alternate explanation to those previously presented by Segal and Meshulam (FEBS Letters 100, 27–32) and Babior (Biochem. Biophys. Res. Comm. 91, 222–226). The paradoxical results previously presented can be explained by recognizing that shortly after activation of resting cells an O2 diffusion layer is established at or near the outer surface of these cells. The presence of this diffusion layer can markedly alter the anticipated stochiometric relationship between trapped and apparent O2 consumed by these cells when they are exposed to trapping agents. |
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