超广谱β-内酰胺酶细菌检测方法及与耐药质粒关系研究

目的：建立一种准确、快速、低廉的检测超广谱β-内酰胺酶（ESBL）细菌方法。方法：以克拉维酸为ESBL的抑制剂、3种第三代头孢菌素（TGC）和氨曲南为底物、某些药敏结果为补充，建立了多底物协同方法，并与E-test方法进行了对照，符合率80%，不符合菌株用质粒提取、细菌转化、接合传递试验进行验证。结果：对116株疑产ESBL细菌用多底物协同方法和E-test方向同时检测，前者比后者阳性率高。结论：该方法准确、快速、价格低廉，特别适于常规应用。

DEVELOPMENT OF A NOVEL TEST IDENTIFYING EXTENDED-SPECTRUMBETA-LACTAMASE PRODUCING STRAINS RELATIVE TO RESISTANT PLASMIDS

Objective:To develop a reliable and time and cost saving assay to identify extended-spectrum beta-lactamase(ESBL) producing strains.Methods:A poly-substrate synergy method(PSSM) was developed with clavulanic acid as ESBL inhibitor,aztreonam and three third-generation cephalosporins as substrates and some established drug sensitive results as references.Results of PSSM were compared with that of E-test and the two methods resulted in conformity with each other in 80% of the strains tested.Strains with different results were further subjected to plasmid extraction,plasmid transformation and conjugation procedures for final identification.Result:Among the 116 strains of ESBL producing suspects tested by both the methods a greater positive rate was achieved by PSSM than that by E-test.Conclusion:PSSM is a reliable,fast and low-cost method for ESBL bacteria identification suitable for routine practice.