| rab5a基因在肿瘤转移中的作用研究 |
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| 引用本文： | 史忠诚,于旸,李钰,傅松滨.rab5a基因在肿瘤转移中的作用研究[J].遗传,2005,27(5):694-698. |
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| 作者姓名： | 史忠诚 于旸 李钰 傅松滨 |
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| 作者单位： | 哈尔滨医科大学医学遗传学研究室，哈尔滨150086 |
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| 基金项目： | 国家自然科学基金，国家自然科学基金，黑龙江省卫生厅科研项目 |
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| 摘 要： | 为研究rab5a基因在肿瘤转移机制中的作用，将该基因稳定转染至低转移肺腺癌细胞系AGZY83-a中，采用Superarray肿瘤转移相关基因微芯片分析rab5a对肿瘤转移相关基因的表达影响，共获得了5个差异表达基因，rab5a基因促进s100a4的表达，同时抑制了nm23a、rac1、cst3、col4a2等基因的表达，并分别在RNA及蛋白水平进行验证，确认rab5a基因影响了肿瘤转移的多个途径，促进了肿瘤细胞转移能力增强。
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| 关 键 词： | 微芯片 rab5a基因 肿瘤转移 |
| 文章编号： | 0253-9772(2005)05-0694-05 |
| 收稿时间： | 2004-11-30 |
| 修稿时间： | 2005-03-29 |
The Roles of rab5a Gene in the Mechanism of Tumor Metastasis |
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| SHI Zhong-Cheng,YU Yang,LI Yu,FU Song-Bin.The Roles of rab5a Gene in the Mechanism of Tumor Metastasis[J].Hereditas,2005,27(5):694-698. |
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| Authors: | SHI Zhong-Cheng YU Yang LI Yu FU Song-Bin |
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| Institution: | Medical Genetics Laboratory of Harbin Medical University, Harbin 150086,China |
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| Abstract: | To study the role of rab5a gene in the mechanism of tumor metastasis, rab5a gene was stable transfected into AGZY83-a cell, a lung adenocarcinoma cell line with low metastatic potential. Superarray was applied to study the effect of rab5a on the differential expression of genes related to tumor metastasis. Five differentially expressed genes were obtained, including one up-regulated gene s100a4 and 4 down-regulated genes, nm23a, rac1, cst3 and col4a2. These results were confirmed at both the RNA and protein levels. We concluded that rab5a promoted tumor metastasis by affecting multiple genes in several pathways involved in tumor metastasis. |
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| Keywords: | rab5a gene tumor metastasis superarray |
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