Isolation, characterization and expression of a cDNA encoding an elongation factor-1α from Porphyra yezoensis (Bangiales, Rhodophyta) |
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| Authors: | Satoru Fukuda Shuuji Ootsuka Yukihiro Kitade Toshiki Watanabe Naotsune Saga † |
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| Institution: | Graduate School of Marine Science and Technology, Tokai University, Shimizu, Shizuoka 424-8610, Japan;Center for Advanced Technology, Tokai University, Shimizu, Shizuoka 424-8610, Japan;Ocean Research Institute, The University of Tokyo, Nakano-ku, Tokyo 164-8639, Japan |
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| Abstract: | We report the isolation, characterization and expression of a cDNA encoding a polypeptide elongation factor‐1α (EF‐1α) from the marine red alga, Porphyra yezoensis. A cDNA clone was isolated from a leafy gametophyte cDNA library and the sequence was analyzed. The clone contained an open reading frame for a protein of 449 amino acids which exhibits sequence similarity to the known EF‐1α. Comparison of the deduced amino acid sequence showed higher similarity to the Porphyra pur‐purea EF‐1αtef‐c (97%) than to the P. purpurea EF‐1αtef‐s (61%). The mRNA was detected both in the leafy gametophyte and the filamentous sporophyte. |
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| Keywords: | cDNA EF-1α gene expression gene structure Porphyra yezoensis Rhodophyta |
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