Dynamics of EF-G interaction with the ribosome explored by classification of a heterogeneous cryo-EM dataset |
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Authors: | Gao Haixiao Valle Mikel Ehrenberg Måns Frank Joachim |
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Affiliation: | Howard Hughes Medical Institute, Health Research, Inc., Empire State Plaza, Albany, NY 12201-0509, USA. |
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Abstract: | A method of supervised classification using two available structure templates was applied to investigate the possible heterogeneity existing in a large cryo-EM dataset of an Escherichia coli 70S ribosome-EF-G complex. Two subpopulations showing the ribosome in distinct conformational states, related by a ratchet-like rotation of the 30S subunit with respect to the 50S subunit, were extracted from the original dataset. The possible presence of additional intermediate states is discussed. |
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Keywords: | Cryo-electron microscopy Reference-based alignment Supervised classification Translation Translocation |
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