Nutrient media for plant parasitic nematodes. V. Amino acid nutrition of Aphelenchoides rutgersi

Nutrition of axenically cultured A. Rutgersi was investigated by deletion and addition of various levels of essential amino acids. Reproduction was lacking when isoleucine, leucine, methionine, phenylalanine, threonine, histidine, tryptophan, and lysine were individually deleted from M-10. Normal reproduction was observed over a range of concentrations but declined to nothing at still higher concentrations of tryptophan and histidine. On the basis of these tests and analyses of both the nematode and host tissue, M-12 was devised and tested. The amino acid group of M-12 contained 7 fewer amino acids and concentrations of another 15 amino acids were adjusted; but no significant differences in reproduction occurred.