Methods to mark termites with protein for mark–release–recapture and mark–capture type studies |
| |
Authors: | J R Hagler P B Baker R Marchosky S A Machtley D E Bellamy |
| |
Institution: | (1) USDA-ARS, Arid-Land Agricultural Research Center, 21881 N. Cardon Lane, Maricopa, AZ 85238, USA;(2) University of Arizona, Tucson, AZ 85721, USA;(3) ED&A Consulting, Buckley, WA 98321, USA |
| |
Abstract: | Studies were conducted to investigate the feasibility of marking the southwestern desert subterranean termite, Heterotermes aureus (Snyder), with rabbit immunoglobulin G (IgG) protein for mark–release–recapture (MRR) and mark–capture type studies. Qualitative
laboratory studies were conducted to determine how long reagent-grade rabbit IgG is retained on or in H. aureus that were marked either externally with a topical spray, internally by feeding them a rabbit IgG-marked food source, or both
internally and externally (double marked). Marked termites were detected by an anti-rabbit IgG enzyme-linked immunosorbent
assay. Data indicated that the termites retained the mark for at least 35 days, regardless of the marking procedure. A second
series of laboratory studies were conducted to determine how fast H. aureus acquire the mark after feeding on cardboard bait that was either sprayed or soaked in different formulations of rabbit IgG.
The IgGs tested were a highly purified and costly reagent grade IgG at 5.0 mg/ml and a less pure and less costly technical
grade rabbit IgG at 1.0 mg/ml. The results showed that termites acquired both marks equally well after exposure to the soaked
cardboard treatment. The advantages and limitations of protein marking termites with rabbit IgG for MRR or mark–capture termite
studies are discussed. |
| |
Keywords: | Heterotermes aureus Protein marking ELISA Trophallaxis Rabbit IgG |
本文献已被 SpringerLink 等数据库收录! |
|