Herpetomonas spp. isolated from tomato fruits (Lycopersicon esculentum) in southern Spain |
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| Authors: | Marín Clotilde Fabre Sandrine Sánchez-Moreno Manuel Dollet Michel |
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| Affiliation: | Instituto de Biotecnología, Departamento de Parasitología, Facultad de Ciencias, Universidad de Granada, C/Severo Ochoa s/n. 18071 Granada, Spain. |
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| Abstract: | A flagellate of the family Trypanosomatidae was isolated from fruits of Lycopersicon esculentum (tomato) in southeastern Spain. The isolate was successfully adapted to in vitro culture in monophasic media. The morphology showed the kinetoplast to be positioned towards the middle of the body, and the typical opistomastigote form characteristic of members of the genus Herpetomonas. Amplification of the mini-exon gene was negative, whilst for the 5S ribosomal rRNA gene the result was positive. The DNA sequence was obtained and its alignment with other trypasomatids, obtained using the BLAST algorithm, suggested it was closely related to Herpetomonas samuelpessoai. |
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| Keywords: | Phytomonas spp. Fruit 5S rRNA SL RNA Herpetomonas |
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