| 软枣猕猴桃悬浮细胞系的建立及其影响因素 |
| |
| 引用本文： | 张喜春,吴绛云.软枣猕猴桃悬浮细胞系的建立及其影响因素[J].植物研究,1991,11(3):77-83. |
| |
| 作者姓名： | 张喜春 吴绛云 |
| |
| 作者单位： | 1. 150040 哈尔滨, 东北农学院生物工程系;2. 150030 哈尔滨, 东北农学院生物工程系 |
| |
| 摘 要： | 以软枣猕猴桃(Actinidia arguta Planch)叶片为试材,以MS为基本培养基,附加生长素(2.4-D、NAA)和玉米素(ZT)成功地诱导出愈伤组织,结果表明:软枣猕猴桃叶片在MS+ZT 1mg/1+2.4-D3mg/1的情况下能诱导出适合于建立悬浮细胞系的愈伤组织。并研究了愈伤组织生长年龄、激素浓度、肌醇和蔗糖浓度四个方面对建立悬浮细胞系的影响。表明愈伤组织以生长10天时接种活细胞率最高;以MS培养基附加ZT1mg/1+2.4-D3mg/1较好;肌醇以200mg/1圆形细胞频率最高;蔗糖以5%最适。
|
| 关 键 词： | 软枣猕猴桃 悬浮细胞系 |
ESTABLISHMENT OF SUSPENSION CELL LINE OF GOOSEBERRY AND INFLUENCEABLE FACTORS |
| |
| Zhang Xi-chun,Wu Jiang-yun.ESTABLISHMENT OF SUSPENSION CELL LINE OF GOOSEBERRY AND INFLUENCEABLE FACTORS[J].Bulletin of Botanical Research,1991,11(3):77-83. |
| |
| Authors: | Zhang Xi-chun Wu Jiang-yun |
| |
| Institution: | 1. Department of forestry, Northeast Fotestry University, Harbin 150040;2. Department of bioengineering, Northeast Agriculture College, Harbin 150030 |
| |
| Abstract: | The leaves of Actinidia arguta Planch were cultured on MS basic med-ium supplemented with 2.4-D or NAA and zeatin in vitro. The callus wereinduced successfullg. The results have been obtained as follows:the callus fitting for the establishment of cell line coulb be obtained from leaves of Act-inidia arguta Planch on MS+ZT1mg/l+2.4-D3mg/l. Tbe highest cell surv-ival rate has been obtained from callus that had been cultured for ten days. The satisfied result have beenobtained on MS+ZT1mg+2.4-D3mg/l.The high-est frequency of round cell is medium with 200mg/l myo-inositol. Suitable concentration of sucrose is 5%. |
| |
| Keywords: | |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《植物研究》浏览原始摘要信息 |
| 点击此处可从《植物研究》下载免费的PDF全文 |
