A sensitive method for the histochemical demonstration of vicinal diols of carbohydrates |
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Authors: | M. Nakamura H. Kitamura K. Yamada |
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Affiliation: | (1) Department of Anatomy, Medical College of Oita, Oita, Japan;(2) Department of Anatomy, Nagoya City University Medical School, Nagoya, Japan |
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Abstract: | Summary A new sensitive method has been established for the histochemical demonstration of vicinal diols of carbohydrates in light microscopy. It consists of a periodic acid-thiocarbohydrazide-silver proteinate (PA-TCH-SP) sequence followed by physical development. The new method is more sensitive than the PA-TCH-SP and periodic acid-Schiff (PAS) methods employed hitherto. Its specificity is sufficient. |
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