玉米优良自交系愈伤组织基因枪转化的研究

利用基因枪将Ｂｔ基因导入玉米Ｅ２８幼胚愈合组织中，在潮霉素浓度为５ｍｇ／Ｌ选择培养基上，经过３５天的继代和分化２次选择及４５天继代继续３次选择后分化再生成植株，ＰＣＲ及Ｓｏｕｔｈｅｒｎ分子杂交结果表明，潮霉素基因及Ｂｔ基因已导入并整合到玉米基因组中，Ｅ２８２次选择的转化频率达到７．８％，显著地高于３次选择２．９％的转化频率，选择至再生的时间缩短２５天；自交系Ｐａｌ３７２次选择的转化频率为１３．３％

Maize Elite Inbred Line Transformed via Callus Bombardment

Elite inbred line E28 calli were differentiated and regenerated after bombardment with Bt gene by particle gun. The transgenic plants were obtained after two or three selections at the concentration of 5mg/L hygromycine during 45 days subculture or/and regeneration. PCR and Southern blot results showed that Bt gene had been tyansferred and integrated into maize genomes. Transgenic frequencies for hyg calli in E28 were 7.8% after two selections and 2.9% in three selections; For Pal37 were 13.3% and 9.3% correspondingly. 25 and 30 days were shortened for the regeneration in E28 and Pal37 respectively. Transformed E28 and Pal37 plants set some seeds.